Peculiarities and systematics of microbial diglycosidases, and their applications in food technology

Diglycosidases are endo-?-glucosidases that hydrolyze the heterosidic linkage of diglycoconjugates, thereby releasing in a single reaction disaccharide and aglycone. Plant diglycosidases belong to glycoside hydrolase family 1 associated with defense mechanisms. Microbial exhibit higher diversity—they families 3, 5, 55—and play catabolic role. As diglycoconjugates widespread environments, so microbial diglycosidases, which allow their utilization as nutritional source carbon recycling. In last 10 years, six have been sequenced, for two them, three-dimensional structure has elucidated. This knowledge allowed identification diverse phylogenetic origin, gave insights into understanding substrate specificity. Here, advances applications reviewed. • Substrate specificity relationships On-going potential discussed.